CD16 antibody

Catalog No. ABIN7304776

The Mouse Monoclonal anti-CD16 antibody is suitable to detect CD16 in samples from Human. It has been validated for FACS, IF and WB.

Quick Overview for CD16 antibody (ABIN7304776)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: CB16

Product Details anti-CD16 Antibody

Purpose: Mouse monoclonal antibody to CD16

Specificity: Recognizes human CD16

Characteristics: Mouse monoclonal antibody to CD16

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD16.

Isotype: IgG1

Application Details

Application Notes: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: CD16B, FCG3, FCGR3, IGFR3, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD antigen CD16b

Gene ID: 2215

UniProt: O75015

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process