CD235a/GYPA antibody

Catalog No. ABIN7687635

The Mouse Monoclonal anti-CD235a/GYPA antibody (Clone HIR2) (ABIN7687635) specifically detects CD235a/GYPA in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD235a/GYPA antibody (ABIN7687635)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HIR2

Product Details anti-CD235a/GYPA Antibody

Purpose: Mouse monoclonal antibody to CD235

Specificity: Recognizes human CD235

Characteristics: Mouse monoclonal antibody to CD235a

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD235a.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a

Background: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD antigen CD235a

Gene ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location