CDK2 antibody

Catalog No. ABIN2718388

The Mouse Monoclonal anti-CDK2 antibody has been validated for WB, IHC, FACS and IF. It is suitable to detect CDK2 in samples from Human.

Quick Overview for CDK2 antibody (ABIN2718388)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 2A5

Product Details anti-CDK2 Antibody

Characteristics: Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human CDK2 (NP_001789) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Molecular Weight: 33.7 kDa

Gene ID: 1017

NCBI Accession: NM_001798

HGNC: 1017

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA