Acetyl-CoA Carboxylase alpha antibody

Catalog No. ABIN271865

The Rabbit Polyclonal anti-Acetyl-CoA Carboxylase alpha antibody is suitable to detect Acetyl-CoA Carboxylase alpha in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for Acetyl-CoA Carboxylase alpha antibody (ABIN271865)

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetyl-CoA Carboxylase alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Acetyl-CoA Carboxylase alpha Antibody

Specificity: This antibody detects endogenous levels of ACC1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/1000-1/5000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Acetyl-CoA Carboxylase alpha

Target: Acetyl-CoA Carboxylase alpha (ACACA)

Alternative Name: Acetyl-CoA Carboxylase 1

Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the ratelimiting step in fatty acid synthesis. Exercise diminishes the activity of acetyl- CoA carboxylase in human muscle. ACCα (ACC1) is the rate-limiting enzyme in the biogenesis of long-chain fatty acids, and ACCβ (ACC2) may control mitochondrial fatty acid oxidation. These two isoforms of ACC control the amount of fatty acids in the cells. The catalytic function of ACCα is regulated by phosphorylation (inactive) and dephosphorylation (active) of targeted Serine residues and by allosteric transformation by citrate or palmitoyl-CoA, which serve as the short-term regulatory mechanism of the enzyme. The gene encoding ACCα, which maps to human chromosome 17, encodes the 265 kDa α form of ACC, which is the major ACC in lipogenic tissues. The catalytic core of ACCβ is homologous to that of ACCα except for an additional peptide of about 150 amino acids at the N-terminus.Synonyms: ACAC, ACACA, ACC1, ACCA

Molecular Weight: approx. 265 kDa

Gene ID: 31

NCBI Accession: NP_942131

UniProt: Q13085

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, Warburg Effect