PLA2G4B antibody

Catalog No. ABIN271921

This Rabbit Polyclonal antibody specifically detects PLA2G4B in WB, IF and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PLA2G4B antibody (ABIN271921)

Target: PLA2G4B (Phospholipase A2, Group IVB (Cytosolic) (PLA2G4B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G4B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PLA2G4B Antibody

Specificity: This antibody detects endogenous levels of c-PLA2 protein. (region surrounding asn499)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: (1/10000approx. 1/20000). Western Blot (1/500approx. 1/1000). Immunohistochemistry (1/50approx. 1/200). Immunofluorescence (1/50approx. 1/200)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PLA2G4B

Target: PLA2G4B (Phospholipase A2, Group IVB (Cytosolic) (PLA2G4B))

Alternative Name: PLA2G4B

Background: Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts, and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.Synonyms: Cytosolic phospholipase A2 beta, Phospholipase A2 group IVB, cPLA2-beta, phospholipase A2 beta

Molecular Weight: approx. 100 kDa

Gene ID: 100137049

NCBI Accession: NP_001108105

UniProt: P0C869

Pathways: ER-Nucleus Signaling, VEGF Signaling