UBR5 antibody

Catalog No. ABIN271946

Product Details anti-UBR5 Antibody

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of EDD protein. (region surrounding Thr14)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Alternative Name: UBR5 / HYD (UBR5 Products)

Synonyms: DD5 antibody, EDD antibody, EDD1 antibody, HYD antibody, 4432411E13Rik antibody, AW549941 antibody, C77315 antibody, D030042K14 antibody, Edd antibody, Edd1 antibody, mKIAA0896 antibody, Dd5 antibody, Rat100 antibody, ubiquitin protein ligase E3 component n-recognin 5 antibody, UBR5 antibody, Ubr5 antibody

Background: EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth (2). EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.Synonyms: E3 ubiquitin-protein ligase, E3 ubiquitin-protein ligase UBR5, EDD, EDD1, HECT domain-containing 1, Hyperplastic discs protein homolog, KIAA0896

Molecular Weight: approx. 309 kDa

Gene ID: 51366

NCBI Accession: NP_056986

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway