NUB1 antibody

Catalog No. ABIN271950

The Rabbit Polyclonal anti-NUB1 antibody (ABIN271950) specifically detects NUB1 in WB and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for NUB1 antibody (ABIN271950)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NUB1 Antibody

Specificity: This antibody detects endogenous levels of NUB1 protein. (region surrounding Tyr601)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Alternative Name: NUB1

Background: NUB1 is a ubiquitin-like protein that controls vital biological events through its conjugation to cullin proteins. NUB1 is a negative regulator of the NEDD8 system that recruits NEDD8 and its conjugates to the proteasome for degradation. It is, therefore a cell growth regulator. The UBA domain of NUB1 is the specific acceptor for the linear ubiquitin precursor. NUB1 is composed of 601 amino acids. It is an interferon-inducible protein and predominantly localizes in the nucleus. NUB1 is specifically expressed in adult human testis, ovary, heart, and skeletal muscle tissues.Synonyms: NEDD8 μLtimate buster 1, NYREN18, Negative regulator of ubiquitin-like proteins 1, Renal carcinoma antigen NY-REN-18

Molecular Weight: approx. 70 kDa

Gene ID: 51667

NCBI Accession: NP_057202

UniProt: Q9Y5A7