UBA7 antibody

Catalog No. ABIN271972

This Rabbit Polyclonal antibody specifically detects UBA7 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for UBA7 antibody (ABIN271972)

Target: UBA7 (Ubiquitin-Like Modifier Activating Enzyme 7 (UBA7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-UBA7 Antibody

Specificity: This antibody detects endogenous levels of UBE1L protein. (region surrounding Glu996)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for UBA7

Target: UBA7 (Ubiquitin-Like Modifier Activating Enzyme 7 (UBA7))

Alternative Name: UBA7 / UBE1L

Background: The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. UBE1 activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. UBE1 is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.Synonyms: UBE2, Ubiquitin-activating enzyme 7, Ubiquitin-activating enzyme E1 homolog, Ubiquitin-like modifier-activating enzyme 7

Molecular Weight: approx. 112 kDa

Gene ID: 7318

NCBI Accession: NP_003326

UniProt: P41226