ALOX5 antibody

Catalog No. ABIN271992

The Rabbit Polyclonal anti-ALOX5 antibody (ABIN271992) specifically detects ALOX5 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ALOX5 antibody (ABIN271992)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALOX5 Antibody

Specificity: This antibody detects endogenous levels of ALOX5 protein. (region surrounding Glu267)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 / LOG5

Background: 5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells. 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis. The cellular localization of 5-LO varies between cell types. In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope. In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane. This intracellular shuttling of 5-LO is dependent on the associationwith various signaling molecules, phosphorylation and the presence of a distinct nuclear localization signal, which is encoded at the amino terminus of 5-LO.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Molecular Weight: approx. 78 kDa

Gene ID: 240

NCBI Accession: NP_000689

UniProt: P09917