Phospholipase C gamma 2 antibody

Catalog No. ABIN272070

The Rabbit Polyclonal anti-Phospholipase C gamma 2 antibody (ABIN272070) specifically detects Phospholipase C gamma 2 in WB and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Phospholipase C gamma 2 antibody (ABIN272070)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Phospholipase C gamma 2 Antibody

Specificity: This antibody detects endogenous levels of PLCG2 protein. (region surrounding asn1211)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western blot 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2

Background: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε). PLC βs are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1181 and 1166 amino acids) of PLC β2 are generated in haematopoietic cells that differ in the carboxyl-terminal sequence implicated in interaction of PLC β enzymes with G αq. The Pleckstrin homology domain of PLC β2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory effect on basal PLC β2 activity. PLC β2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-2, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, Phospholipase C-gamma-2

Molecular Weight: approx. 148 kDa

Gene ID: 5336

NCBI Accession: NP_002652

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling