SOX9 antibody

Catalog No. ABIN272082

Cited in 3+ publications. The Rabbit Polyclonal anti-SOX9 antibody (ABIN272082) specifically detects SOX9 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for SOX9 antibody (ABIN272082)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SOX9 Antibody

Specificity: This antibody detects endogenous levels of SOX-9 protein. (region surrounding Gln175).

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000Western Blot: 1/500approx. 1/1000Immunohistochemistry (Paraffin): 1/50approx. 1/200Immunofluorescence: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SOX9 antibody (ABIN272082)

Ichikawa, Watahiki, Nampo, Nose, Yamamoto, Irie, Mishima, Maki: "Differences in the developmental origins of the periosteum may influence bone healing." in: Journal of periodontal research, Vol. 50, Issue 4, pp. 468-78, (2016) (PubMed).

Zhang, Ding, Nie, Li-Ling, Zhang, Zhang, Chen, Li, Ding: "Association of hsa-miR‑145 overexpression in human testicular cells with male infertility." in: Molecular medicine reports, Vol. 11, Issue 6, pp. 4365-72, (2015) (PubMed).

Nagappan, Park, Park, Kim, Hong, Kang, Zhang, Kim, Lee, Won, Kim: "Proteomic analysis of differentially expressed proteins in vitamin C-treated AGS cells." in: BMC biochemistry, Vol. 14, pp. 24, (2014) (PubMed).

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.Synonyms: CMD1, CMPD1, SRA1, SRY (sex determining region Y)-box 9, SRY-box 9, Transcription factor SOX-9

Molecular Weight: approx. 56 kDa

Gene ID: 6662

NCBI Accession: NP_000337

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development