WASP antibody

Catalog No. ABIN272092

This Rabbit Polyclonal antibody specifically detects WASP in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for WASP antibody (ABIN272092)

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-WASP Antibody

Specificity: This antibody detects endogenous levels of WASP protein. (region surrounding Glu284)

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sdium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for WASP

Target: WASP (WAS) (Wiskott-Aldrich Syndrome (Eczema-thrombocytopenia) (WAS))

Alternative Name: WAS / IMD2

Background: The Wiskott-Aldrich syndrome (WAS) is a disorder that results from a monogenic defect that has been mapped to the short arm of the X chromosome at Xp11.23. WAS is characterized by thrombocytopenia, eczema, defects in cell-mediated and humoral immunity and a propensity for lymphoproliferative disease. The gene that is mutated in the syndrome encodes a 53 kDa proline-rich protein of unknown function designated WAS protein (WASP). A clue to WASP function came from the observation that T cells from affected males had an irregular cellular morphology and a disarrayed cytoskeleton suggesting the involvement of WASP in cytoskeletal organization. Close examination of the WASP sequence revealed a putative Cdc42/Rac interacting domain, homologous with those found in PAK65 and ACK. Subsequent investigation has shown WASP to be a true downstream effector of Cdc42.Synonyms: WASp, Wiskott-Aldrich syndrome protein

Molecular Weight: approx. 53 kDa

Gene ID: 7454

NCBI Accession: NP_000368

UniProt: P42768