Survivin antibody

Catalog No. ABIN272115

The Rabbit Polyclonal anti-Survivin antibody (ABIN272115) specifically detects Survivin in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Survivin antibody (ABIN272115)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Survivin Antibody

Specificity: This antibody detects endogenous levels of Survivin protein. (region surrounding asn111)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: IAP4 / BIRC5

Background: The baculovirus protein p35 inhibits virally-induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FASmediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivinmicrotubule interactions occurs.Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat-containing protein 5, Survivin

Molecular Weight: approx. 20 kDa

Gene ID: 332

NCBI Accession: NP_001012270

UniProt: O15392

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding