CKM antibody

Catalog No. ABIN272157

Product Details anti-CKM Antibody

Target: CKM (Creatine Kinase, Muscle (CKM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Creatine Kinase M protein. (region surrounding Tyr14)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: CKMM (CKM Products)

Synonyms: CKMM antibody, M-CK antibody, Ckmm antibody, MCK antibody, ckmm antibody, m-ck antibody, CKM antibody, CK-M antibody, cb51 antibody, ckm antibody, mck antibody, wu:fa28d05 antibody, ckm3 antibody, wu:fb55e09 antibody, zgc:64204 antibody, zgc:92070 antibody, creatine kinase, M-type antibody, creatine kinase, muscle antibody, creatine kinase, M-type L homeolog antibody, creatine kinase, muscle a antibody, creatine kinase, muscle b antibody, CKM antibody, Ckm antibody, ckm.L antibody, ckm antibody, ckma antibody, ckmb antibody

Background: Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKM isoform, predominant in skeletal muscle and heart tissue, is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.Synonyms: CKM, Creatine kinase M chain, Creatine kinase M-type, Creatine kinase MM, M-CK

Molecular Weight: approx. 43 kDa

Gene ID: 1158

NCBI Accession: NP_001815

UniProt: P06732