GALE antibody

Catalog No. ABIN2721595

The Mouse Monoclonal anti-GALE antibody is suitable to detect GALE in samples from Human, Rat, Dog and Monkey. It has been validated for WB and IHC.

Quick Overview for GALE antibody (ABIN2721595)

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Reactivity: Human, Rat, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GALE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 1C4

Product Details anti-GALE Antibody

Characteristics: Homo sapiens UDP-galactose-4-epimerase (GALE), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human GALE (NP_000394) produced in HEK293T cell.

Isotype: IgG1

Application Details

Application Notes: WB 1:500~2000, IHC 1:150,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for GALE

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Alternative Name: GALE

Background: This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Molecular Weight: 38.1 kDa

Gene ID: 2582

NCBI Accession: NM_000403

HGNC: 2582

Pathways: Response to Water Deprivation, Cellular Glucan Metabolic Process