NCBP2 antibody

Catalog No. ABIN272203

Product Details anti-NCBP2 Antibody

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of CBP20 protein. (region surrounding Arg21)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for NCBP2

Target: NCBP2 (Nuclear Cap Binding Protein Subunit 2 (NCBP2))

Alternative Name: NCBP2 (NCBP2 Products)

Synonyms: CBP20 antibody, CG12357 antibody, Dmel\\CG12357 antibody, 20kDa antibody, 5930413E18Rik antibody, AA536802 antibody, AI843301 antibody, C79367 antibody, CHUNP6917 antibody, zgc:92592 antibody, CBC2 antibody, NIP1 antibody, ATCBP20 antibody, CAP-binding protein 20 antibody, MLN1.12 antibody, MLN1_12 antibody, Ncbp2 antibody, cap binding protein 20 antibody, nuclear cap binding protein subunit 2 antibody, nuclear cap binding protein subunit 2, 20kDa antibody, nuclear cap binding protein subunit 2 L homeolog antibody, nuclear cap-binding protein subunit 2 antibody, CAP-binding protein 20 antibody, Nuclear cap-binding protein subunit 2 antibody, Cbp20 antibody, Ncbp2 antibody, ncbp2 antibody, NCBP2 antibody, ncbp2.L antibody, CBP20 antibody, ncbp-2 antibody

Background: In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay (NMD), a process which eliminates mRNAs, and prematurely terminates translation. CBP80-bound mRNA undergoes a pioneer round of translation before CBP80-CBP20 are replaced by eIF4E, and Upf2 and Upf3 proteins.Synonyms: CBP20, Nuclear cap-binding protein subunit 2, PIG55

Molecular Weight: approx. 18 kDa

Gene ID: 22916

NCBI Accession: NP_001036005

UniProt: P52298

Pathways: Ribonucleoprotein Complex Subunit Organization, Methionine Biosynthetic Process