SRY antibody

Catalog No. ABIN272215

Product Details anti-SRY Antibody

Target: SRY (Sex Determining Region Y (SRY))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRY antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of SRY protein. (region surrounding Leu80)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for SRY

Target: SRY (Sex Determining Region Y (SRY))

Alternative Name: SRY / TDF (SRY Products)

Background: SRY (sex-determining region Y protein) is a transcriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.Synonyms: Sex-determining region Y protein, Testis-determining factor

Molecular Weight: approx. 37 kDa

Gene ID: 100130809

NCBI Accession: NP_003131

UniProt: Q05066