NFIA antibody
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- Target See all NFIA Antibodies
- NFIA (Nuclear Factor I/A (NFIA))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NFIA antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects endogenous levels of NF-1 protein. (region surrounding Gln34)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product NFIA Primary Antibody
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- Application Notes
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- NFIA (Nuclear Factor I/A (NFIA))
- Alternative Name
- NFIA (NFIA Products)
- Synonyms
- NFIA antibody, CTF antibody, NF-I/A antibody, NF1-A antibody, NFI-A antibody, NFI-L antibody, si:ch211-88d2.2 antibody, wu:fq27e07 antibody, zgc:158351 antibody, CNFI-A antibody, cNFI-A3 antibody, 1110047K16Rik antibody, 9430022M17Rik antibody, NF1A antibody, nuclear factor I A antibody, nuclear factor I/A antibody, NFIA antibody, nfia antibody, Nfia antibody
- Background
- NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.Synonyms: CCAAT-box-binding transcription factor, CTG, NF-I/A, NFI-A, Nuclear factor 1 A-type, TGGCA-binding protein
- Molecular Weight
- approx. 55 kDa
- Gene ID
- 4774
- NCBI Accession
- NP_001128145
- UniProt
- Q12857
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