14-3-3 theta antibody

Catalog No. ABIN272267

The Rabbit Polyclonal anti-14-3-3 theta antibody is suitable to detect 14-3-3 theta in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for 14-3-3 theta antibody (ABIN272267)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-14-3-3 theta Antibody

Specificity: This antibody detects endogenous levels of 14-3-3 θ protein. (region surrounding Gln67)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 Protein theta

Background: 14-3-3 proteins regulate many cellular processes relevant to cancer biology,notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activitiesSynonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

Molecular Weight: approx. 28 kDa

Gene ID: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction