CDC37 antibody

Catalog No. ABIN272280

Product Details anti-CDC37 Antibody

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC37 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Cdc37 protein. (region surrounding Phe259)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for CDC37

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Alternative Name: CDC37 (CDC37 Products)

Synonyms: CG12019 antibody, CPDR-1 antibody, CPDR-2 antibody, DROCPDR antibody, Dmel\\CG12019 antibody, E(sev)3B antibody, cdc37 antibody, Afu4g10010 antibody, P50CDC37 antibody, p50 antibody, p50Cdc37 antibody, CG12019 gene product from transcript CG12019-RA antibody, cell division cycle 37 homolog antibody, Hsp90 co-chaperone Cdc37 antibody, Cdc37p antibody, cell division cycle 37 antibody, Cdc37 antibody, cdc37 antibody, AFUA_4G10010 antibody, CDC37 antibody

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.Synonyms: CDC37A, Hsp90 chaperone protein kinase-targeting subunit, Hsp90 co-chaperone Cdc37, p50Cdc37

Molecular Weight: approx. 44 kDa

Gene ID: 11140

NCBI Accession: NP_008996

UniProt: Q16543