ACTN3 antibody
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- Target See all ACTN3 Antibodies
- ACTN3 (Actinin, alpha 3 (ACTN3))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ACTN3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous levels of Actinin-α3 protein. (region surrounding Glu25)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product ACTN3 Primary Antibody
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- Application Notes
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- ACTN3 (Actinin, alpha 3 (ACTN3))
- Alternative Name
- alpha-Actinin-3 / ACTN3 (ACTN3 Products)
- Synonyms
- CG8953 antibody, Dmel\\CG8953 antibody, ORF1 antibody, acta-3 antibody, dabp antibody, ACTN2 antibody, actn3 antibody, actnl antibody, zgc:63559 antibody, fb95c03 antibody, wu:fb95c03 antibody, wu:fc11d03 antibody, zgc:77243 antibody, actinin alpha 3 (gene/pseudogene) antibody, actinin alpha 3 antibody, alpha actinin 3 antibody, actinin alpha 3a antibody, actinin alpha 3 (gene/pseudogene) L homeolog antibody, alpha-actinin-3 antibody, actinin alpha 3b antibody, ACTN3 antibody, Actn3 antibody, actn3a antibody, actn3.L antibody, actn3 antibody, LOC100439754 antibody, actn3b antibody
- Background
- The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.Synonyms: Alpha actinin skeletal muscle isoform 3, F-actin cross-linking protein
- Molecular Weight
- approx. 103 kDa
- Gene ID
- 89
- NCBI Accession
- NP_001095
- UniProt
- Q08043
- Pathways
- Cell-Cell Junction Organization
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