ADD3 antibody
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- Target See all ADD3 Antibodies
- ADD3 (Adducin 3 (Gamma) (ADD3))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ADD3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Adducin γ protein. (region surrounding Pro452)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product ADD3 Primary Antibody
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- Application Notes
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- ADD3 (Adducin 3 (Gamma) (ADD3))
- Alternative Name
- gamma-Adducin (ADD3) (ADD3 Products)
- Synonyms
- gamma-adducin antibody, add3 antibody, MGC89418 antibody, ADD3 antibody, DKFZp459E0630 antibody, ADDL antibody, AI463285 antibody, R75380 antibody, adducin 3 (gamma) antibody, adducin 3 antibody, adducin 3 L homeolog antibody, ADD3 antibody, add3 antibody, add3.L antibody, Add3 antibody
- Background
- Adducins are a family of cytoskeleton proteins encoded by three genes (α, β and γ). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin α is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin α and Adducin γ is ubiquitous in contrast to the restricted expression of Adducin β. Adducin β is expressed at high levels in brain andhematopoietic tissues, such as bone marrow in humans, and spleen in mice.Synonyms: ADDL, Adducin 3, Adducin-like protein 70
- Molecular Weight
- approx. 82 kDa
- Gene ID
- 120
- NCBI Accession
- NP_001112
- UniProt
- Q9UEY8
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