ERCC4 antibody

Catalog No. ABIN272306

The Rabbit Polyclonal anti-ERCC4 antibody is suitable to detect ERCC4 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for ERCC4 antibody (ABIN272306)

Target: ERCC4 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 (ERCC4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ERCC4 Antibody

Specificity: This antibody detects endogenous levels of ERCC4/XPF protein.

Cross-Reactivity (Details): Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000approx. 1/10000. Western blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ERCC4

Target: ERCC4 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 (ERCC4))

Alternative Name: ERCC4

Background: Xeroderma Pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer, and it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a 115 kDa protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5' incisions during DNA repair. The ERCC1-XPF endonuclease preferentially cleaves one strand of DNA between duplex and singlestranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process.Synonyms: DNA excision repair protein ERCC-4, DNA repair endonuclease XPF, DNA repair protein complementing XP-F cells, ERCC-4, ERCC11, XPF, Xeroderma pigmentosum group F-complementing protein

Molecular Weight: approx. 103 kDa

Gene ID: 2072

NCBI Accession: NP_005227

UniProt: Q92889

Pathways: DNA Damage Repair