POLR2E antibody

Catalog No. ABIN2729316

This anti-POLR2E antibody is a Mouse Monoclonal antibody detecting POLR2E in WB, IHC, IF and FACS. Suitable for Human, Mouse, Rat, Monkey and Dog.

Quick Overview for POLR2E antibody (ABIN2729316)

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Reactivity: Human, Mouse, Rat, Monkey, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLR2E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 3B5

Product Details anti-POLR2E Antibody

Characteristics: Homo sapiens polymerase (RNA) II (DNA directed) polypeptide E, 25 kDa (POLR2E)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.

Isotype: IgG2a

Application Details

Application Notes: WB 1:500~2000, IHC 1:150, FLOW 1:100, IF: 1:100

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for POLR2E

Target: POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))

Alternative Name: POLR2E

Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

Molecular Weight: 24.4 kDa

Gene ID: 5434

NCBI Accession: NM_002695

HGNC: 5434

Pathways: Regulatory RNA Pathways