Actin antibody (N-Term)

Catalog No. ABIN2738124

The Mouse Monoclonal anti-Actin antibody has been validated for WB, IHC, ELISA, IF, IHC (p), IP and FACS. It is suitable to detect Actin in samples from Human, Rat, Mouse, Chicken, Rabbit, Dog, Cow, Monkey, Pig, Guinea Pig, Cat, Baboon, Sheep and Goat.

Quick Overview for Actin antibody (N-Term) (ABIN2738124)

Target: Actin

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Dog, Cow, Monkey, Pig, Guinea Pig, Cat, Baboon, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 1A4

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Specificity: Human Actin

Purification: Protein G purified

Immunogen: An N-Terminal decapeptide of the alpha smooth muscle isoform.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin. No special pretreatment is required for the immunohistochemical staining of FFPE tissue. ELISA: Order BSA-free format.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 1 month or -20°C for longer. Avoid freeze/thaw cycles.

Target Details for Actin

Target: Actin