BTLA antibody (APC)

Catalog No. ABIN2738632

This anti-BTLA antibody is a Mouse Monoclonal antibody detecting BTLA in FACS and ELISA. Suitable for Human.

Quick Overview for BTLA antibody (APC) (ABIN2738632)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTLA antibody is conjugated to APC

Application: Flow Cytometry (FACS), ELISA

Clone: ANC6E9

Product Details anti-BTLA Antibody (APC)

Specificity: Antibody from clone ANC6E9 binds to CD272 on the cell surface of human PBMC in Flow cytometry, and to recombinant CD272-muIg in EIA.

Purification: Protein A purified

Immunogen: Recombinant extracellular human CD272.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 human peripheral blood mononuclear cells (PBMC) per tube were washed and incubated 45 minutes on ice with 80 μL of anti-CD272/APC at a 1:50 dilution (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to Mouse IgG1/APC Isotype Control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD272 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Alternative Name: BTLA / CD272

Background: Name/Gene ID: BTLA

Synonyms: BTLA, BTLA1, B and T lymphocyte associated, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, CD272 antigen

Gene ID: 151888

Pathways: Cancer Immune Checkpoints