CD3 epsilon antibody

Catalog No. ABIN2739340

The Hamster Monoclonal anti-CD3 epsilon antibody (Clone 145-2C11) (ABIN2739340) specifically detects CD3 epsilon in FACS, WB, IP and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for CD3 epsilon antibody (ABIN2739340)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody

Specificity: Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

Purification: Protein G purified

Immunogen: Mouse BM10-37 T cell membrane prep.

Type of Immunogen: Membrane preparation

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IP, WB

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD3 antibody diluted to 10 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33 % sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C, avoid freeze-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway