CD3 epsilon antibody

Catalog No. ABIN2739341

This Rabbit Polyclonal antibody specifically detects CD3 epsilon in FACS, WB and IP. It exhibits reactivity toward Human, Mouse, Rat, Pig, Monkey, Cow, Horse, Rabbit, Sheep, Hamster and Orang-Utan.

Quick Overview for CD3 epsilon antibody (ABIN2739341)

Target: CD3 epsilon (CD3E)

Reactivity: Human, Mouse, Rat, Pig, Monkey, Cow, Horse, Rabbit, Sheep, Hamster, Orang-Utan

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-CD3 epsilon Antibody

Specificity: Mouse CD3e.

Purification: Protein A purified

Immunogen: Amino acids ERPPPVPNPDYEP (156-168). Percent identity by BLAST analysis: Human, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Rabbit, Pig (100%), Chimpanzee, Gorilla, Ferret, Cat, Chicken, Dog (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo (0.5 - 1 μg/10 x10⁶), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway