CD74 antibody

Catalog No. ABIN2739637

The Mouse Monoclonal anti-CD74 antibody (Clone LN-2) (ABIN2739637) specifically detects CD74 in FACS, WB, IF, IHC, ELISA, IP and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for CD74 antibody (ABIN2739637)

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD74 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LN-2

Product Details anti-CD74 Antibody

Specificity: Human CD74

Purification: Protein G purified

Immunogen: SU-DHL-4 lymphoma cells.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 / CLIP

Background: Name/Gene ID: CD74

Synonyms: CD74, CD74 antigen, DHLAG, HLADG, HLA-DR-gamma, Ia-associated invariant chain, p33, MHC HLA-DR gamma chain, Ia-GAMMA

Gene ID: 972

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints