CD8 alpha antibody (APC)

Catalog No. ABIN2739735

The Rat Monoclonal anti-CD8 alpha antibody is suitable to detect CD8 alpha in samples from Mouse. It has been validated for FACS and ELISA.

Quick Overview for CD8 alpha antibody (APC) (ABIN2739735)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to APC

Application: Flow Cytometry (FACS), ELISA

Clone: 53-6-72

Product Details anti-CD8 alpha Antibody (APC)

Specificity: Mouse CD8.

Purification: Protein G purified

Immunogen: Mouse thymocytes/splenocytes.

Type of Immunogen: Cells

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-Mouse CD8/APC at a 1:50 dilution (2 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 9 % sub population of the cells stained positive with a mean shift of 2.59 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A / CD8 alpha

Background: Name/Gene ID: CD8A

Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2

Gene ID: 925

Pathways: TCR Signaling