CTLA4 antibody (FITC)

Catalog No. ABIN2740167

The Mouse Monoclonal anti-CTLA4 antibody is suitable to detect CTLA4 in samples from Human. It has been validated for FACS, ELISA and BR.

Quick Overview for CTLA4 antibody (FITC) (ABIN2740167)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: ANC152-2-8H5

Product Details anti-CTLA4 Antibody (FITC)

Specificity: Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells.

Purification: Protein A purified

Immunogen: Mouse IgG1, kappa.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Block, ELISA, Flo

Usage: Anti-CD152/FITC was tested for binding to CD152-muIg-coated 4u beads in FACS (Inquire). Two x 105 CD152-muIg coated beads per tube were spun down and incubated 45 minutes at 4oC with 80 μL of anti-CD152/FITC at a 1:50 dilution (10 μg/mL). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 2.03 log10 fluorescent units when compared to a Mouse IgG1/FITC Isotype control at a similar concentration. Binding was blocked when beads were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD152 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C

Storage Comment: Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4 / CD152

Background: Name/Gene ID: CTLA4

Synonyms: CTLA4, CD152, CELIAC3, CD152 antigen, CTLA-4, Celiac disease 3, IDDM12, GRD4, GSE, CD152 isoform

Gene ID: 1493

Pathways: Cancer Immune Checkpoints