EGF antibody (Biotin)

Catalog No. ABIN2740488

The Mouse Monoclonal anti-EGF antibody is suitable to detect EGF in samples from Human. It has been validated for WB, ELISA, ICC and IF.

Quick Overview for EGF antibody (Biotin) (ABIN2740488)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 3A8

Product Details anti-EGF Antibody (Biotin)

Specificity: The anti-EGF antibody (biotinylated conjugate of clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11). Typical dilutions for sandwich ELISA: Coat = 1-3 μg/mL and Detection = 0.125-0.5 μg/mL.

Purification: Protein A purified

Immunogen: Recombinant human EGF.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.12 - 0.25 μg/mL), ICC (1:50), IF (1:50), WB (1:1000 - 1:5000)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 4 % BSA, proprietary preservative.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gene ID: 1950

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation