EpCAM antibody (PE)

Catalog No. ABIN2740721

This Mouse Monoclonal antibody specifically detects EpCAM in FACS and ELISA. It exhibits reactivity toward Human.

Quick Overview for EpCAM antibody (PE) (ABIN2740721)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is conjugated to PE

Application: Flow Cytometry (FACS), ELISA

Clone: ANC8D4

Product Details anti-EpCAM Antibody (PE)

Specificity: Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS.

Purification: Protein A purified

Immunogen: Recombinant human CD326(EpCAM).

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo

Usage: Five x 105 cultured human SW480 cells were harvested by trypsiniaztion, washed and pre incubated ~5 minutes with 20 μL of 250 μg/mL human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD326/R-PE at a dilution factor of 1:50 (10 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL antiCD326 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: ESA / EPCAM

Background: Name/Gene ID: EPCAM

Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

Gene ID: 4072