LRRC32 antibody (FITC)
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- Target See all LRRC32 Antibodies
- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This LRRC32 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS), ELISA
- Specificity
- Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
- Purification
- Protein A purified
- Immunogen
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Recombinant human GARP.
Type of Immunogen: Recombinant protein - Clone
- ANC8C9
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product LRRC32 Primary Antibody
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- Application Notes
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Approved: ELISA, Flo
Usage: : (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 μg/mL). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1 % sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- Target
- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
- Alternative Name
- LRRC32 (LRRC32 Products)
- Synonyms
- D11S833E antibody, GARP antibody, AI426318 antibody, D11S833Eh antibody, D7H11S833E antibody, EG434215 antibody, Garp antibody, leucine rich repeat containing 32 antibody, LRRC32 antibody, Lrrc32 antibody
- Background
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Name/Gene ID: LRRC32
Synonyms: LRRC32, D11S833E, GARP, Garpin - Gene ID
- 2615
- Pathways
- Activated T Cell Proliferation
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