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LRRC32 antibody

LRRC32 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal ANC8C9 unconjugated
Catalog No. ABIN2742631
  • Target See all LRRC32 Antibodies
    LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
    Reactivity
    • 23
    • 9
    • 8
    • 1
    Human
    Host
    • 21
    • 9
    • 1
    • 1
    Mouse
    Clonality
    • 22
    • 10
    Monoclonal
    Conjugate
    • 18
    • 6
    • 3
    • 2
    • 2
    • 1
    This LRRC32 antibody is un-conjugated
    Application
    • 16
    • 14
    • 13
    • 7
    • 3
    • 3
    • 2
    • 1
    Flow Cytometry (FACS), ELISA
    Specificity
    Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
    Purification
    Protein A purified
    Immunogen
    Recombinant human GARP.

    Type of Immunogen: Recombinant protein
    Clone
    ANC8C9
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product LRRC32 Primary Antibody
  • Application Notes
    Approved: ELISA, Flo

    Usage: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 4°C, avoid freeze-thaw cycles.
  • Target
    LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
    Alternative Name
    LRRC32 (LRRC32 Products)
    Background
    Name/Gene ID: LRRC32

    Synonyms: LRRC32, D11S833E, GARP, Garpin
    Gene ID
    2615
    Pathways
    Activated T Cell Proliferation
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