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LRRC32 antibody

LRRC32 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal ANC8C9 unconjugated
Catalog No. ABIN2742631
  • Target See all LRRC32 Antibodies
    LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
    Reactivity
    • 26
    • 10
    • 9
    • 1
    Human
    Host
    • 20
    • 12
    • 1
    • 1
    Mouse
    Clonality
    • 21
    • 13
    Monoclonal
    Conjugate
    • 18
    • 7
    • 4
    • 2
    • 2
    • 1
    This LRRC32 antibody is un-conjugated
    Application
    • 16
    • 16
    • 13
    • 6
    • 4
    • 3
    • 2
    • 1
    Flow Cytometry (FACS), ELISA
    Specificity
    Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
    Purification
    Protein A purified
    Immunogen
    Recombinant human GARP.

    Type of Immunogen: Recombinant protein
    Clone
    ANC8C9
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product LRRC32 Primary Antibody
  • Application Notes
    Approved: ELISA, Flo

    Usage: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.
    Preservative
    Gentamicin sulfate
    Precaution of Use
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 4°C, avoid freeze-thaw cycles.
  • Target
    LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
    Alternative Name
    LRRC32 (LRRC32 Products)
    Synonyms
    D11S833E antibody, GARP antibody, AI426318 antibody, D11S833Eh antibody, D7H11S833E antibody, EG434215 antibody, Garp antibody, leucine rich repeat containing 32 antibody, LRRC32 antibody, Lrrc32 antibody
    Background
    Name/Gene ID: LRRC32

    Synonyms: LRRC32, D11S833E, GARP, Garpin
    Gene ID
    2615
    Pathways
    Activated T Cell Proliferation
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