TAG-72 antibody

Catalog No. ABIN2744653

This Mouse Monoclonal antibody specifically detects TAG-72 in IHC (p), IHC, FACS, IF, WB, IP and ELISA. It exhibits reactivity toward Human, Rat, Cow, Dog and Hamster.

Quick Overview for TAG-72 antibody (ABIN2744653)

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivity: Human, Rat, Cow, Dog, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAG-72 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP), ELISA

Clone: B72-3

Product Details anti-TAG-72 Antibody

Specificity: Human TAG-72

Purification: Protein G purified

Immunogen: The membrane-enriched fraction of a human breast carcinoma liver metastasis.

Type of Immunogen: Membrane preparation

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Flo (0.5 - 1 μg/10 x10⁶), IF (1 - 2 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: This antibody recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma. ELISA: Order BSA-free format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for TAG-72

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72

Background: Name/Gene ID: TAG-72

Synonyms: TAG-72