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Vimentin antibody (AA 435-466)

The Rabbit Polyclonal anti-Vimentin antibody has been validated for WB, IHC and IHC (p). It is suitable to detect Vimentin in samples from Human, Mouse, Rat, Cow, Chicken, Monkey, Sheep, Hamster, Guinea Pig, Chimpanzee and Gibbon.
Catalog No. ABIN2745278

Quick Overview for Vimentin antibody (AA 435-466) (ABIN2745278)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human, Mouse, Rat, Cow, Chicken, Monkey, Sheep, Hamster, Guinea Pig, Chimpanzee, Gibbon

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

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    AA 435-466

    Specificity

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .

    Purification

    Immunogen affinity purified

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Distilled water

    Concentration

    Lot specific

    Buffer

    Lyophilized from 5 mg BSA, 0.9 mg sodium chloride, 0.2 mg sodium phosphate, 0.05 mg sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    avoid freeze thaw cycles

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Alternative Name

    Vimentin

    Background

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    Gene ID

    7431

    Pathways

    Caspase Cascade in Apoptosis
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