BMF antibody (AA 13-27, AA 112-126)

Catalog No. ABIN2745519

Product Details anti-BMF Antibody

Target: BMF (Bcl2 Modifying Factor (BMF))

Binding Specificity: AA 13-27, AA 112-126

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This BMF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Specificity: Recognizes an epitope within the peptide sequence aa 13-27 of mouse and rat Bmf. Detects bands of ~25 kDa and ~30 kDa by Western blot, corresponding to endogenous Bmf and BmfL.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

No Cross-Reactivity: Human

Cross-Reactivity (Details): Does not cross-react with human Bmf.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 and aa 112-126 of mouse Bmf.

Clone: 17A9

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for BMF

Target: BMF (Bcl2 Modifying Factor (BMF))

Alternative Name: Bmf (BMF Products)

Background: Bmf (Bcl-2-modifying factor) belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homo-dimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Bmf contains a single Bcl-2 homology domain 3 (BH3), and has been shown to interact with other Bcl-2 proteins and functions as an apoptotic activator. Two different start sites can give rise to two proteins of 25 or 30 kDa. It is found to be sequestered to myosin V motors by its association with dynein light chain 2 (DLC2), which may be important for sensing intracellular damage and triggering apoptosis.

UniProt: Q91ZE9