Caspase 12 antibody (AA 183-205)

Catalog No. ABIN2745524

This Rat Monoclonal antibody specifically detects Caspase 12 in WB, ELISA and FACS. It exhibits reactivity toward Mouse.

Quick Overview for Caspase 12 antibody (AA 183-205) (ABIN2745524)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 12G6

Product Details anti-Caspase 12 Antibody

Binding Specificity: AA 183-205

Purpose: anti-Caspase-12 (mouse), mAb (12G6)

Specificity: Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51 kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.

Cross-Reactivity (Details): Does not cross-react with mouse caspase-1 or -11.

Characteristics: Monoclonal Antibody. Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51 kDa by Western blot. Does not cross-react with mouse caspase-1 or -11. Isotype: Rat IgG1kappa. Clone: 12G6. Applications: ELISA, FACS, WB. Liquid. In PBS containing 0.02 % sodium azide. Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51 kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12

Background:

Alternate Names/Synonyms: CASP-12

Product Description: Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51 kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.

UniProt: O08736

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response