Vestigial (VG) (phosphorylated) antibody

Catalog No. ABIN2747809

Product Details

Target: Vestigial (VG)

Binding Specificity: phosphorylated

Reactivity: Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: This antibody detects a single band of approximately 54 kDa in ABIN1041056 samples.

Cross-Reactivity: Fly (Calliphora), Mouse (Murine), Rat (Rattus)

Characteristics: Phospho specific Vstgl-selective antibodies were generated against purified Glutamate receptor 1 (PVstgl) protein. PVstgl-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. The supplier will also provide limited quantities of antigenic blocking protein for Pvstgl antibody. Antibodies to PVstgl (PVstgl -140AP) will label 54 kDa protein in Western blot positive control samples for PVstgl and several other tissues. FabGennix Inc. will also conjugate antibodies with secondary enzymes (alk-Pase or HRP) or fluorescent probes upon request at nominal cost. Limited quantities of antigenic blocking peptide is also available, please inquire before placing the orders.

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Vestigial protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: Vg, VG, vg21, Dmel/CG3830, CG3830

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details

Target: Vestigial (VG)

Alternative Name: Protein Vestigial (VG Products)

Synonyms: CG3830 antibody, Dmel\CG3830 antibody, VG antibody, Vg antibody, vg21 antibody, vestigial antibody, vg antibody

Background: Phosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies, monospecific for a target protein that is phosphorylated on a specific tyrosine, threonine, or serine residue.The fly Drosophila melanogaster is one of the most intensively studied organisms in biology used for the investigation of many developmental and cellular processes common to higher eukaryotes, including humans. The entire fly genome encodes approximately 13,600 genes with vast functional diversity. The dorsal ventral patterning in vertebrate embryos is regulated by members of TGF-beta family of growth and differentiation factors. Regulatory genes in insects regulate the conserved signal transduction pathways and provide organ-specific information that is crucial in the development of organs such as wings, eyes and legs. One such gene in Drosophila Melanogaster (fruit flies) is vestigial (vg) which is approximately a 54 kDa protein. Vg, by interacting with other nuclear proteins, is implicated in determining which thoracic imaginal disc cells will form wings and halters. Vg, via its interaction with transcription factor scalloped (sd), regulates wing-specific gene expression in Drosophila. This vg-sd interaction forms an active complex that binds to specific DNA sequences and regulates gene expression in cooperation with several signaling pathways. Vg gene is activated by dorsoventral organizing signals, and is induced by long-range signaling protein, Decapentaplegic (Dpp). Vg is expressed in the developing wing discs only, and so is known as a selector protein for wing identity and development. A decrease in vg function shifts the dorso-ventral boundary cells of the disc to a cell death sensitive state, and loss of vg function eliminates wing and haltere formation. However, only the TEA domain of scalloped is required for wing development & the rest of the protein is dispensable. Phosphorylation of the serine/threonine-rich domain is crucial in regulating cytokine-specific cell differentiation. Vestigial-like 2 (Vgll2), a vertebrates homolog of Vg, is specifically expressed in skeletal muscle. Over-expression of Vgll2 enhances myotube formation whereas Vgll2 knockdown blocks myogenic differentiation, demonstrating the important role of Vgll2 in skeletal muscle differentiation. Casein kinase II (CKII) is conserved as phosphorylation site (Serine 96) in all vestigial-like (Vgll) proteins. Bacterially-expressed purified GST-Vgll2 protein is selectively phosphorylated by CKII in vitro. Immunocytochemistry results imply that phosphorylation of the serine 96 residue is necessary for the Vgll2-based myogenic differentiation. Inactivation of Drosophila tumor suppressor gene causes excessive proliferation and neoplastic growth. Metastatic tumors of Drosophila provide a model to identify genes that are involved in the metastatic process.

NCBI Accession: NP_523723

UniProt: Q26366