Interferon gamma antibody

Catalog No. ABIN2748934

Cited in 3+ publications. The Mouse Monoclonal anti-Interferon gamma antibody (Clone DB-1) (ABIN2748934) specifically detects Interferon gamma in WB, ELISA, IHC, ELISpot, Neut and ICS. The antibody is reactive with Rat samples.

Quick Overview for Interferon gamma antibody (ABIN2748934)

Target: Interferon gamma (IFNG)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), ELISpot, Neutralization (Neut), Intracellular Staining (ICS)

Clone: DB-1

Product Details anti-Interferon gamma Antibody

Cross-Reactivity: Mouse (Murine)

Characteristics: The mouse anti-rat IFN-γ monoclonal antibody . In Western blot analysis the DB-1 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-γ with moderate activity. Clone DB-1 has shown not to bind with rat IFN-α and IFN-β nor human IFNs. Further, biotinyated DB-1 antibody or biotinylated polyclonal antibody to rat IFN-γ as detector antibody . The DB-1 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining .

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile distilled water

Storage: 4 °C

References for anti-Interferon gamma antibody (ABIN2748934)

Verma, Boyd, Robinson, Plain, Tran, Hall: "Interleukin-12p70 prolongs allograft survival by induction of interferon gamma and nitric oxide production." in: Transplantation, Vol. 82, Issue 10, pp. 1324-33, (2007) (PubMed).

Darji, Sileghem, Heremans, Brys, De Baetselier: "Inhibition of T-cell responsiveness during experimental infections with Trypanosoma brucei: active involvement of endogenous gamma interferon." in: Infection and immunity, Vol. 61, Issue 7, pp. 3098-102, (1993) (PubMed).

van der Meide, Dubbeld, Vijverberg, Kos, Schellekens: "The purification and characterization of rat gamma interferon by use of two monoclonal antibodies." in: The Journal of general virology, Vol. 67 ( Pt 6), pp. 1059-71, (1986) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Gene ID: 25712

NCBI Accession: NP_620235

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy