TNFRSF10A antibody

Catalog No. ABIN2749024

Cited in 1 publication. The Mouse Monoclonal anti-TNFRSF10A antibody (Clone DR-4-02) (ABIN2749024) specifically detects TNFRSF10A in FACS, IP, ICC and Func. The antibody is reactive with Human samples.

Quick Overview for TNFRSF10A antibody (ABIN2749024)

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF10A antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Clone: DR-4-02

Product Details anti-TNFRSF10A Antibody

Purpose: Anti-Hu CD261 Purified Low Endotoxin

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.

Isotype: IgG1

Application Details

Application Notes: Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/mL in cultivation medium, final concentration of TRAIL: 20-200 ng/mL, it is recommended to add the antibody 15 min before addition of TRAIL.
Flow cytometry: Recommended dilution: 2-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-TNFRSF10A antibody (ABIN2749024)

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Target Details for TNFRSF10A

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: CD261

Background: TNF receptor superfamily member 10a,TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).,DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1

Gene ID: 8797

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity