CD40 Ligand antibody

Catalog No. ABIN2749183

This anti-CD40 Ligand antibody is a Mouse Monoclonal antibody detecting CD40 Ligand in FACS and ICC. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 5+ publications.

Quick Overview for CD40 Ligand antibody (ABIN2749183)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 24-31

Product Details anti-CD40 Ligand Antibody

Purpose: Anti-Hu CD154 Purified

Specificity: The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: human CD154 fusion protein

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-6 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD40 Ligand antibody (ABIN2749183)

Brams, Black, Padlan, Hariharan, Leonard, Chambers-Slater, Noelle, Newman: "A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation." in: International immunopharmacology, Vol. 1, Issue 2, pp. 277-94, (2001) (PubMed).

Berner, Wolf, Hummel, Müller, Reuss-Borst: "Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis." in: Annals of the rheumatic diseases, Vol. 59, Issue 3, pp. 190-5, (2000) (PubMed).

Barnhart, Ford, Bhushan, Song, Covey: "A polymorphic CD40 ligand (CD154) molecule mediates CD40-dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions." in: Immunology, Vol. 99, Issue 1, pp. 54-61, (2000) (PubMed).

Grammer, McFarland, Heaney, Darnell, Lipsky: "Expression, regulation, and function of B cell-expressed CD154 in germinal centers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4150-9, (1999) (PubMed).

Kornbluth, Kee, Richman: "CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive beta-chemokines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5205-10, (1998) (PubMed).

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154

Background: CD40 ligand,CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.,CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

Gene ID: 21947

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints