PVRL2 antibody

Catalog No. ABIN2749202

This Mouse Monoclonal antibody specifically detects PVRL2 in FACS, IP and IHC (fro). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PVRL2 antibody (ABIN2749202)

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PVRL2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: R2-525

Product Details anti-PVRL2 Antibody

Purpose: Anti-Hu CD112 Purified

Specificity: The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: NIH/3T3 cells transfected with human Nectin-2

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-PVRL2 antibody (ABIN2749202)

Hou, Ge, Zheng, Wei, Sun, Tian: "CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain." in: The Journal of biological chemistry, Vol. 289, Issue 10, pp. 6969-77, (2014) (PubMed).

Target Details for PVRL2

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: CD112

Background: Nectin cell adhesion molecule 2,CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,NECTIN2, HVEB, PRR2, PVRL2, PVRR2

Gene ID: 5819

UniProt: Q92692

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization