Actin antibody (N-Term)

Catalog No. ABIN2763613

This Rabbit Polyclonal antibody specifically detects Actin in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Actin antibody (N-Term) (ABIN2763613)

Target: Actin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Characteristics: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Purification: Antigen Immunoaffiinity Purification

Immunogen: Synthetic peptide derived from the N-terminal of the beta-actin protein.

Isotype: Ig Fraction

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 μg/mL) and immunohistochemsitry on paraffin-embedded tissues (10 μg/mL), Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Actin

Target: Actin

UniProt: P60709