FECH antibody (Middle Region)

Catalog No. ABIN502157

The Rabbit Polyclonal anti-FECH antibody is suitable to detect FECH in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio) and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for FECH antibody (Middle Region) (ABIN502157)

Target: FECH (Ferrochelatase (FECH))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FECH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FECH Antibody

Binding Specificity: Middle Region

Sequence: KRSEVVILFS AHSLPMSVVN RGDPYPQEVS ATVQKVMERL EYCNPYRLVW

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%, Yeast: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against FECH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FECH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 423 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FECH

Target: FECH (Ferrochelatase (FECH))

Alternative Name: FECH

Background: FECH is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: EPP, FCE
Protein Interaction Partner: PPP2R1A, UBC, NEDD8, MDM2, FBXO6, gag-pol, COPS5, COPS6, CUL3, ELAVL1, MINOS1, MME, USP42, USP20, ABCB7, FECH,
Protein Size: 423

Molecular Weight: 42 kDa

Gene ID: 2235

NCBI Accession: NM_000140, NP_000131

UniProt: P22830

Pathways: Transition Metal Ion Homeostasis