OTC antibody (N-Term)

Catalog No. ABIN2773791

Cited in 4+ publications. The Rabbit Polyclonal anti-OTC antibody (ABIN2773791) specifically detects OTC in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Horse, Pig, Sheep, Rabbit, Goat and Guinea Pig samples.

Quick Overview for OTC antibody (N-Term) (ABIN2773791)

Target: OTC (Ornithine Carbamoyltransferase (OTC))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Pig, Sheep, Rabbit, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-OTC Antibody

Binding Specificity: N-Term

Purpose: OTC Antibody - N-terminal region

Sequence: AFRNGHNFMV RNFRCGQPLQ NKVQLKGRDL LTLKNFTGEE IKYMLWLSAD

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against OTC. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human OTC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107402

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-OTC antibody (ABIN2773791)

Hallows, Yu, Smith, Devries, Devires, Ellinger, Someya, Shortreed, Prolla, Markley, Smith, Zhao, Guan, Denu: "Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction." in: Molecular cell, Vol. 41, Issue 2, pp. 139-49, (2011) (PubMed).

Walters, Wallace: "Urea cycle gene expression is suppressed by PFOA treatment in rats." in: Toxicology letters, Vol. 197, Issue 1, pp. 46-50, (2010) (PubMed).

Yu, Lin, Yao, Huang, Lei, Xiong, Zhao, Guan: "Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13669-75, (2009) (PubMed).

Tanaka, Wada, Maruyama, Tanaka, Takikawa, Komatsu: "Hyperammonemia-induced encephalopathy due to ornithine transcarbamylase deficiency in an adult woman: identification of novel missense mutations." in: Journal of gastroenterology, Vol. 40, Issue 1, pp. 106-7, (2005) (PubMed).

Target Details for OTC

Target: OTC (Ornithine Carbamoyltransferase (OTC))

Alternative Name: OTC

Background:

Background Information: OTC is a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

Gene Name: Ornithine carbamoyltransferase

Alternative Symbols: OCTD, OTCD

Protein Name: Ornithine carbamoyltransferase, mitochondrial

Molecular Weight: 40 kDa

Gene ID: 5009

NCBI Accession: NP_000522

UniProt: P00480