SEC63 antibody (C-Term)

Catalog No. ABIN2773888

The Rabbit Polyclonal anti-SEC63 antibody (ABIN2773888) specifically detects SEC63 in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Cow and Horse samples.

Quick Overview for SEC63 antibody (C-Term) (ABIN2773888)

Target: SEC63 (SEC63 Homolog (SEC63))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC63 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SEC63 Antibody

Binding Specificity: C-Term

Purpose: SEC63 Antibody - C-terminal region

Sequence: WWLYIADRKE QTLISMPYHV CTLKDTEEVE LKFPAPGKPG NYQYTVFLRS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against SEC63. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SEC63

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111229

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SEC63

Target: SEC63 (SEC63 Homolog (SEC63))

Alternative Name: SEC63

Background:

Background Information: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. SEC63 and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. SEC63 is an integral membrane protein located in the rough ER.The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC62 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.

Gene Name: SEC63 homolog (S. cerevisiae)

Alternative Symbols: ERdj2, PCLD2, SEC63L, DNAJC23, PRO2507

Protein Name: Translocation protein SEC63 homolog

Molecular Weight: 88kDa

Gene ID: 11231

NCBI Accession: NP_009145

UniProt: Q9UGP8

Pathways: ER-Nucleus Signaling