ME3 antibody (N-Term)

Catalog No. ABIN2773921

The Rabbit Polyclonal anti-ME3 antibody has been validated for WB. It is suitable to detect ME3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio) and Sheep.

Quick Overview for ME3 antibody (N-Term) (ABIN2773921)

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ME3 Antibody

Binding Specificity: N-Term

Sequence: PGPARPVPLK KRGYDVTRNP HLNKGMAFTL EERLQLGIHG LIPPCFLSQD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ME3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ME3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 604 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ME3

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Alternative Name: ME3

Background: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. ME3 is a mitochondrial NADP(+)-dependent isoform. Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Alias Symbols: FLJ34862, NADP-ME
Protein Interaction Partner: UBC, TRAF4,
Protein Size: 604

Molecular Weight: 67 kDa

Gene ID: 10873

NCBI Accession: NM_001014811, NP_001014811

UniProt: Q16798

Pathways: Warburg Effect