DCI antibody (N-Term)

Catalog No. ABIN2773922

The Rabbit Polyclonal anti-DCI antibody is suitable to detect DCI in samples from Human and Cow. It has been validated for WB.

Quick Overview for DCI antibody (N-Term) (ABIN2773922)

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCI antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DCI Antibody

Binding Specificity: N-Term

Purpose: DCI Antibody - N-terminal region

Sequence: ALVASVRVPA RVLLRAGARL PGAALGRTER AAGGGDGARR FGSQRVLVEP

Predicted Reactivity: Cow: 87%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against DCI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DCI

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48556-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for DCI

Target: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Alternative Name: DCI

Background:

Background Information: DCI is a member of the hydratase/isomerase superfamily. It is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined.This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined.

Gene Name: Enoyl-CoA delta isomerase 1

Alternative Symbols: DCI

Protein Name: Enoyl-CoA delta isomerase 1, mitochondrial

Molecular Weight: 29kDa

Gene ID: 1632

NCBI Accession: NP_001910

UniProt: P42126